The portrait captures John with his husband David Furnish along with their two children and dogs.
The portrait taken by photographer Catherine Opie is available to view from Tuesday, with the portrait marking the opening of the exhibition 'Catherine Opie: To Be Seen.'
"To have our family photographed by Catherine Opie and on display at the National Portrait Gallery is a huge honour. We are huge admirers of her work and proud to have her beautiful and poignant images in our collection," John and Furnish said.
Speaking about the portrait, photographer Opie said: "I arrived at Elton and David’s house three days before Christmas. I met the boys and the dogs and after a great lunch together, I made this family portrait of them in their library. It is truly an honour to photograph Elton, David, Zachary and Elijah. For me, it represents the humanity of what family can be."
The National Portrait Gallery director, Victoria Siddall said that the collection exists to share portraits of people "who have shaped the history and culture of the UK."
"The people on our walls, and the stories we tell about them, are a source of inspiration for the millions of people who come through our doors, and particularly the many young people who visit every year," Siddall continued.
Siddall went on to explain that the portrait not only celebrates Johns musical achievements but also Furnish's and his humanitarian and philanthropic work and the family they have built together.
